Histochemical analysis of wild rabbits’ ovaries, oviducts, and folliculogenesis N. Al-
DOI:
https://doi.org/10.31210/spi2025.28.04.32Keywords:
histochemical, wild rabbits, ovaryAbstract
The reproductive system of wild rabbits is highly specific to support their prolific breeding capacity. The histological characteristics of the ovaries and oviducts in wild rabbits, focusing on follicular development, oviductal structure, and functional adaptations are reviewed in this article. Eight wild rabbits were studied in this investigation, using the routine H&E stain and Masson’s trichrome staining for detected collagen fibers. The current research studied the histological features in wild rabbits and found that the animals had lobulated ovaries lined by tall cuboidal germinal layer, followed by thin layer of tunica albuginea. The ovaries exhibit distinct cortical and medullary regions with various stages of follicular growth. The primordial follicles are present and located near the tunica albuginea. In addition, the mature follicles are surrounded by a few fibers (theca interna). Primordial follicles, which are composed of a single layer of flattened granulosa cells surrounding an oocyte, appear to be abundant in the present research. The primary follicles also comprise a single layer of cuboidal granulosa cells enveloping the oocyte, whereas the secondary follicles have a variety of granulosa cells forming, and a theca layer that differentiates into theca externa (fibrous) and theca interna. The stroma of the ovaries has a large amount of collagen fibers and their increase in the cortical region with the decrease in medulla. The oviduct is divided into infundibulum, ampulla, and isthmus, each with unique epithelial and glandular features. The histological structure of the oviduct includes mucosa with ciliated and secretory cells aiding ovum transport and nourishment. Beneath lies the lamina propria for support and immunity. Muscularis mucosae enables movement via contractions. The outer serosa, part of the peritoneum, provides structural support with simple squamous epithelium. Hormonal regulation and seasonal variations also influence these structures. Understanding these histological features provides insights into the reproductive potential of wild rabbits.
References
Sharma, M., Karikalan, M., Dandapat, P., Asok Kumar, M., Beena, V., Chandra Mohan, S., Ilayaraja, S., Mathur, A., Bhawal, A., Pawde, A. M., & Sharma, A. K. (2022). Tuberculosis in free-ranging and captive wild animals: Pathological and molecular diagnosis with histomorphological differentiation of granulomatous lesions. Microbial Pathogenesis, 172, 105752. https://doi.org/10.1016/j.micpath.2022.105752
Comizzoli, P., Songsasen, N., Hagedorn, M., & Wildt, D. E. (2012). Comparative cryobiological traits and requirements for gametes and gonadal tissues collected from wildlife species. Theriogenology, 78 (8), 1666–1681. https://doi.org/10.1016/j.theriogenology.2012.04.008
Ebedes, H. (1976). Anthrax epizootics in wildlife in the Etosha National Park, South West Africa. Wildlife Diseases, 519–526. https://doi.org/10.1007/978-1-4757-1656-6_5
McNamara, T. S. (2016). Wildlife pathology studies and how they can inform public health. ILAR Journal, 56 (3), 306–311. https://doi.org/10.1093/ilar/ilv043
Cooper, J. E. (2002). Diagnostic pathology of selected diseases in wildlife. Revue Scientifique et Technique de l’OIE, 21 (1), 77–89. https://doi.org/10.20506/rst.21.1.1320
Jones, K. E., & Safi, K. (2011). Ecology and evolution of mammalian biodiversity. Philosophical Transactions of the Royal Society B: Biological Sciences, 366 (1577), 2451–2461. https://doi.org/10.1098/rstb.2011.0090
Lu, H., Ma, L., Zhang, Y., Feng, Y., Zhang, J., & Wang, S. (2022). Current animal model systems for ovarian aging research. Aging and Disease, 13 (4), 1183. https://doi.org/10.14336/ad.2021.1209
Wildt, D. E., & Wemmer, C. (1999). Sex and wildlife: the role of reproductive science in conservation. Biodiversity & Conservation, 8 (7), 965–976. https://doi.org/10.1023/a:1008813532763
Comizzoli, P., & Ottinger, M. A. (2021). Understanding reproductive aging in wildlife to improve animal conservation and human reproductive health. Frontiers in Cell and Developmental Biology, 9, 680471. https://doi.org/10.3389/fcell.2021.680471
Abidu-Figueiredo, M., Xavier-Silva, B., Cardinot, T. M., Babinski, M. A., & Chagas, M. A. (2008). Celiac artery in New Zealand rabbit: anatomical study of its origin and arrangement for experimental research and surgical practice. Pesquisa Veterinária Brasileira, 28 (5), 237–240. https://doi.org/10.1590/s0100-736x2008000500002
Tortereau, A., Howroyd, P., & Lorentsen, H. (2013). Onset of Puberty and normal histological appearances of the reproductive organs in peripubertal female göttingen minipigs. Toxicologic Pathology, 41 (8), 1116–1125. https://doi.org/10.1177/0192623313482777
Gad, S. C. (Eds.). (2016). Animal Models in Toxicology. CRC Press. https://doi.org/10.1201/b18705
Tammam, O. Y., Taha, A. A., & El-Sherif, M. W. (2019). Optimization of Xylazine-Ketamine anesthetic dose in mice suffering chronic liver injury. Journal of Anesthesia & Critical Care: Open Access, 11 (1), 6–8. https://doi.org/10.15406/jaccoa.2019.11.00403
Mustafa, K. A., Al-Zubaidy, M. H., & Al-Baggou, B. Kh. (2025). Adverse developmental and behavioral effects of imidacloprid in mice. Iraqi Journal of Veterinary Sciences, 39 (2), 199–205. https://doi.org/10.33899/ijvs.2025.155589.4043
Al-Haaik, A. G., & Mahmood, S. K. (2024). Immunohistochemical localization and distribution of Cajal cell in the intestine of rabbit. Iraqi Journal of Veterinary Sciences, 38 (4), 817–822. https://doi.org/10.33899/ijvs.2024.150004.3676
Bradbury, P., & Gordon, K. (1990). Connective tissues and stains. In: J. D. Bancroft, A. Stevens (eds.) The Theory and Practice of Histological Techniques. 3rd Ed. (pp. 119–142). Avon: The Bath Press.
Nishida, T., & Nishida, N. (2006). Reinstatement of "germinal epithelium" of the ovary. Reproductive Biology and Endocrinology, 4, 42. https://doi.org/10.1186/1477-7827-4-42
Bertoldo, M. J., Holyoake, P. K., Evans, G., & Grupen, C. G. (2012). Seasonal variation in the ovarian function of sows. Reproduction, Fertility and Development, 24 (6), 822–834. https://doi.org/10.1071/rd11249
McGee, E. A., & Hsueh, A. J. W. (2000). Initial and cyclic recruitment of ovarian follicles. Endocrine Reviews, 21 (2), 200–214. https://doi.org/10.1210/edrv.21.2.0394
Skinner, M. K. (2005). Regulation of primordial follicle assembly and development. Human Reproduction Update, 11 (5), 461–471. https://doi.org/10.1093/humupd/dmi020
Erickson, G. F., & Shimasaki, S. (2000). The role of the oocyte in folliculogenesis. Trends in Endocrinology & Metabolism, 11 (5), 193–198. https://doi.org/10.1016/s1043-2760(00)00249-6
Hirshfield, A. N. (1991). Development of follicles in the mammalian ovary. International Review of Cytology, 43–101. https://doi.org/10.1016/s0074-7696(08)61524-7
Jabara, S., & Lambert-Messerlian, G. (2006). Ovarian structure and function. In J. F. Strauss & R. L. Barbieri (Eds.) Yen and Jaffe’s Reproductive Endocrinology (6th ed.). Saunders.
Oktem, O., & Urman, B. (2010). Understanding follicle growth in vivo. Human Reproduction, 25 (12), 2944–2954. https://doi.org/10.1093/humrep/deq275
Kinnear, H. M., Tomaszewski, C. E., Chang, A. L., Moravek, M. B., Xu, M., Padmanabhan, V., & Shikanov, A. (2020). The ovarian stroma as a new frontier. Reproduction, 160 (3), R25–R39. https://doi.org/10.1530/rep-19-0501
Araki, Y. (2003). Formation and structure of mammalian ovaries. Introduction to Mammalian Reproduction, 141–153. https://doi.org/10.1007/978-1-4615-0273-9_9
Buhi, W. C., Alvarez, I. M., & Kouba, A. J. (2020). Oviductal regulation of fertilization and early embryonic development. Bioscientifica Proceedings. https://doi.org/10.1530/biosciprocs.15.0021
Verhage, H. G., Bareither, M. L., Jaffe, R. C., & Akbar, M. (1979). Cyclic changes in ciliation, secretion and cell height of the oviductal epithelium in women. American Journal of Anatomy, 156 (4), 505–521. https://doi.org/10.1002/aja.1001560405
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