The effect of citrate Se on the biochemical parameters of rabbit blood
DOI:
https://doi.org/10.31210/spi2026.29.01.36Keywords:
selenium, rabbits, nanotechnology, catalase, superoxide dismutase, lipid hydroperoxides, malondialdehyde (MDA) productsAbstract
The research was conducted at a private rabbit farm on young rabbits of the Termon breed (Hylla). The rabbits were grouped according to similar characteristics (age, body weight, clinical condition) into groups of 5–6 animals, with an average body weight of 1,000–1,200 g. Selenium citrate was obtained from Nanomaterials and Nanotechnologies LLC, Kyiv. At 45 days of age, the animals were divided into four groups: a control group and three experimental groups. The rabbits in the control group were fed a standard pelleted feed and given unlimited access to water in accordance with current regulatory requirements for the feeding and housing of rabbits in commercial settings. The experimental group, in addition to and drinking water, received an aqueous solution of nanotechnology-based selenium citrate at a concentration of 50 μg Se/L throughout the day. Accordingly, the second experimental group received selenium citrate at a concentration of 100 μg Se/L; the third experimental group received selenium citrate at a concentration of 200 μg Se/L. During the experimental period (on days 15 and 30 of the study), daily monitoring was conducted to assess survival, growth rate, and development. Parameters of the platelet component of hemostasis (platelet count, mean platelet volume, hematocrit, platelet distribution width) and the antioxidant system (GPL, TBA-active products, SOD, catalase) in rabbit blood plasma were determined using standard methods. The administration of selenium citrate at concentrations of 50, 100, and 200 μg Se/L resulted in an increase in platelet parameters in the blood of the experimental groups of rabbits, accompanied by a decrease in lipid peroxidation products. The addition of selenium citrate to the rabbits’ diet was associated with increased activity of antioxidant defense enzymes in their blood throughout the study period, with a statistically significant increase in catalase activity (P<0.05–0.001) in the blood of rabbits in the experimental groups on days 15 and 30 of the study and superoxide dismutase (P<0.05) in animals in the experimental groups on day 30 of the study. The inclusion of selenium citrate in the rabbits’ diet contributed to a reduction in blood cholesterol levels: by day 15, cholesterol levels in animals from experimental groups II and III were 1.6 and 1.4 times lower, respectively (P<0.05), and on day 30 – by 22.8 % and 30.1 % (P<0.05) compared to the control group.
References
1. Abdelsalam, M., & Fathi, M. (2023). Improving productivity in rabbits by using some natural feed additives under hot environmental conditions — A review. Animal Bioscience, 36 (4), 540–554. https://doi.org/10.5713/ab.22.0354
2. Banuelos, G. S., Lin, Z.-Q., & Caton, J. (2023). Editorial: Selenium in soil-plant-animal systems and its essential role for human health. Frontiers in Plant Science, 14, 1237646. https://doi.org/10.3389/fpls.2023.1237646
3. Chandimali, N., Bak, S. G., Park, E. H., Lim, H.-J., Won, Y.-S., Kim, E.-K., Park, S.-I., & Lee, S. J. (2025). Free radicals and their impact on health and antioxidant defenses: a review. Cell Death Discovery, 11 (1), 19. https://doi.org/10.1038/s41420-024-02278-8
4. Drutel, A., Archambeaud, F., & Caron, P. (2013). Selenium and the thyroid gland: more good news for clinicians. Clinical Endocrinology, 78 (2), 155–164. https://doi.org/10.1111/cen.12066
5. Pro zakhyst tvaryn vid zhorstokoho povodzhennia [On the protection of animals from cruelty]: Zakon Ukrainy No. 3447-IV. (2006). Vidomosti Verkhovnoi Rady Ukrainy, (27), Art. 230. [in Ukrainian]
6. Fedoruk, R. S., Tesarivska, U. I., Kovalchuk, I. I., Tsap, M. M., Kaplunenko, V. H., Koleschuk, O. I., & Khrabko, M. I. (2021). Biological effects of iodine, selenium, sulfur citrates in broiler chickens. Regulatory Mechanisms in Biosystems, 12 (3), 523–530. https://doi.org/10.15421/022172
7. Hashem, N. M., Abd El-Hady, A. M., & Hassan, O. A. (2017). Inclusion of phytogenic feed additives comparable to vitamin E in diet of growing rabbits: Effects on metabolism and growth. Annals of Agricultural Sciences, 62 (2), 161–167. https://doi.org/10.1016/j.aoas.2017.11.003
8. Iskra, R. Ya. (2012). Funktsionalnyi stan systemy antyoksydantnoho zakhystu v pechintsi ta skeletnykh miazakh kroliv za dii riznykh doz khromu [Functional state of the antioxidant defense system in the liver and skeletal muscles of rabbits under the action of different doses of chromium]. Visnyk Kyivskoho Natsionalnoho Universytetu Imeni Tarasa Shevchenka. Biolohiia, 60, 4–6. [in Ukrainian]
9. Duntas, L. H. (2010). Selenium and the thyroid: A close-knit connection. The Journal of Clinical Endocrinology & Metabolism, 95 (12), 5180–5188. https://doi.org/10.1210/jc.2010-0191
10. Lesyk, Ya. V., Fedoruk, R. S., & Kropyvka, S. I. (2012). Hematolohichni pokaznyky ta antyoksydantnyi status orhanizmu kroliv za vypoiuvannia tsytratu i khlorydu khromu [Hematological parameters and antioxidant status of the rabbit body under the influence of chromium citrate and chloride]. Biolohiia Tvaryn, 14 (1–2), 141–149. [in Ukrainian]
11. López-Alonso, M., Rivas, I., & Miranda, M. (2025). Trace mineral imbalances in global health: Challenges, biomarkers, and the role of serum analysis. Nutrients, 17 (13), 2241. https://doi.org/10.3390/nu17132241
12. Moustafa, K.-E. M. El., EL-Hosseiny, H. M., Shaheen, G. F., El-Kotamy, E. M., Ghoniem, A. E., Younan, G. E., El-Nahrawy, M. M., Farag, M. E., & Mohamed, M. S. (2024). Impact of different forms of selenium supplementation on growth and physiological performance of New Zealand white rabbits. Tropical Animal Health and Production, 56 (4), 131. https://doi.org/10.1007/s11250-024-03970-8
13. Oguro, H. (2019). The roles of cholesterol and its metabolites in normal and malignant hematopoiesis. Frontiers in Endocrinology, 10, 204. https://doi.org/10.3389/fendo.2019.00204
14. Palazzetti, S., Rousseau, A.-S., Richard, M.-J., Favier, A., & Margaritis, I. (2004). Antioxidant supplementation preserves antioxidant response in physical training and low antioxidant intake. British Journal of Nutrition, 91 (1), 91–100. https://doi.org/10.1079/bjn20031027
15. Kosinov, M. V., & Kaplunenko, V. H. (2009). Sposib otrymannia karboksylativ metaliv. Nanotekhnolohiia otrymannia karboksylativ metaliv [Method for obtaining metal carboxylates. Nanotechnology for obtaining metal carboxylates] (Ukrainian Patent No. 38391). State Intellectual Property Service of Ukraine. https://sis.nipo.gov.ua/uk/search/detail/276279/ [in Ukrainian]
16. Petrovska, I., Salyha, Yu., & Vudmaska, I. (2022). Statystychni metody v biolohichnykh doslidzhenniakh [Statistical methods in biological research]. Ahrarna Nauka. [in Ukrainian]
17. Placha, I., Pogány Simonová, M., & Lauková, A. (2022). Natural feed additives and novel approaches for healthy rabbit breeding. Animals, 12 (16), 2111. https://doi.org/10.3390/ani12162111
18. Popoiu, S., Teodoru, A.-M., Levandovschi, N., & Coman, C. (2021). Hematological and biochemical dynamics of rabbits and guinea pigs used for scientific purposes at Cantacuzino Institute, Bucharest. Revista Română de Medicină Veterinară, 31 (2), 69–80.
19. Postoi, R. V., Karpovskyi, V. I., & Postoi, V. V. (2019). Cholesterol and triacylglycerols content in blood of dry sows depending on peculiarities of nervous system activity. Scientific Reports of the National University of Life and Environmental Sciences of Ukraine, 15 (5), 138–147. https://doi.org/10.31548/dopovidi2019.05.014
20. Rublenko, M. V., & Andriievskyi, V. H. (2008). Ahrehatsiia trombotsytiv u sobak ta svynei u normi [Platelet aggregation in dogs and pigs in norm]. Visnyk Poltavskoi Derzhavnoi Ahrarnoi Akademii, 2, 118–120. [in Ukrainian]
21. Sela-Culang, I., Kunik, V., & Ofran, Y. (2013). The structural basis of antibody-antigen recognition. Frontiers in Immunology, 4, 302. https://doi.org/10.3389/fimmu.2013.00302
22. Serra, V., Castrica, M., Agradi, S., Curone, G., Vigo, D., Di Giancamillo, A., Modina, S. C., Riva, F., Balzaretti, C. M., De Bellis, R., Brecchia, G., & Pastorelli, G. (2023). Antioxidant activity of different tissues from rabbits fed dietary bovine colostrum supplementation. Animals, 13 (5), 850. https://doi.org/10.3390/ani13050850
23. Skowron, M., Zalejska-Fiolka, J., Błaszczyk, U., Chwalińska, E., Owczarek, A., & Birkner, E. (2018). Antioxidant enzyme activities in rabbits under oxidative stress induced by high fat diet. Journal of Veterinary Research, 62 (2), 199–205. https://doi.org/10.2478/jvetres-2018-0019
24. Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory research methods in biology, animal husbandry and veterinary medicine]. Spolom. [in Ukrainian]
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 О. В. Проданчук
This work is licensed under a Creative Commons Attribution 4.0 International License.
