Діагностична ефективність способів копроовоскопії під час виявлення яєць стронгілід травного тракту у овець
DOI:
https://doi.org/10.31210/spi2026.29.02.09Keywords:
parasitology, sheep, strongylidoses, intravital diagnostics, coproovoscopy, efficiencyAbstract
Gastrointestinal nematodoses, in particular strongylidoses, lead to economic losses in livestock farms due to the negative impact of parasites on the body of ruminants, where, depending on the type of strongylids, they can cause anemia, diarrhea, weight loss, reduced productivity, and death. For laboratory diagnostics of strongylidoses of the digestive tract in animals, well-known flotation methods based on the microscopic detection of eggs are used. However, their sensitivity varies significantly, which can lead to a decrease in the effectiveness of diagnostic measures. The aim of the research was to investigate, in a comparative aspect, the sensitivity and effectiveness of known methods of coproovoscopy for strongylidoses of the digestive tract of sheep. Four flotation methods were tested under experimental conditions, namely: Dakhnoʼs (with bischofite), Sorokovaʼs (with a mixture of calcium and ammonium nitrate solutions), Starodubʼs (with a mixture of sugar and calcium nitrate solutions), and Petrenkoʼs (with a mixture of calcium nitrate, sugar, and table salt solutions) with exposure times of 5, 10, and 15 minutes. The conducted studies established that all tested methods of coproovoscopy are sensitive and allow for the detection of 100 % of positive samples. At the same time, the most effective method, which allows for the isolation of the largest number of strongylid eggs from the digestive tract of sheep, was the Petrenkoʼs method. With its use and an exposure of stool samples for 5 minutes, the intensity of strongylidosic invasion exceeded the effectiveness of the Dakhnoʼs method by 32.3 %, Sorokovaʼs by 19.4 %, and Starodubʼs by 8.8 %. With exposures of 10 and 15 minutes, the effectiveness of the Petrenkoʼs method exceeded the indicators of animal invasion by strongylids of the digestive tract obtained using the Dakhnoʼs method by 36.1 % and 30.2 %, Sorokovaʼs by 26.3 % and 22.5 %, and Starodubʼs by 19.5 % and 16.9 %, respectively. The obtained research results allow us to recommend the method where a mixture of calcium nitrate, sugar, and table salt solutions is used as a flotation fluid for effective intravital laboratory diagnostics of strongylidoses of the digestive tract in sheep.
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