Clinical and biochemical substantiation of Iruvetol cream-gel application in the treatment of accidental purulent wounds in horses
DOI:
https://doi.org/10.31210/spi2026.29.02.12Keywords:
horses, accidental purulent wounds, local therapy, Iruvetol ointment, planimetry, biochemical parameters, blood serum, enzyme activityAbstract
The article presents a study on the prevalence of accidental surgical trauma in horses and provides a clinical, biochemical, and planimetric substantiation of Iruvetol ointment efficacy in purulent wound treatment. The research was conducted in 2025–2026 at Vladus Riding Club (Mykhailivka, Poltava region) on two groups of horses with accidental infected wounds. Wound injuries were diagnosed in 24.1 % of the examined population. Trauma demonstrated a clear seasonal dependence: in geldings and foals, injuries occurred exclusively in spring (100.0 %), while in mares, 66.7 % of wounds were recorded in spring and 33.3 % in summer. Planimetric measurements proved that local application of Iruvetol ointment provides a pronounced anti-edematous effect, reducing the marginal edema zone area by 11.4 % on the second day and by 32.5 % on the third day relative to the baseline level, which significantly exceeded the edema reduction rate in the control group (26.3 %, p<0.01). Cleansing of the wound cavity from necrotic detritus in the experimental group was 13.3 % faster than in the control group. On the twenty-first day, the final wound area with Iruvetol was 2.8 times smaller than the baseline and 11.9 % smaller than in the control group. Biochemical serum analysis revealed that baseline activity of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in sick animals exceeded the norm by 32.0 %, 38.4 %, and 22.8–50.4 %, respectively. On the fifth day, the experimental group showed a decrease in alanine aminotransferase activity by 27.0 %, aspartate aminotransferase by 10.9 %, and alkaline phosphatase by 17.3 %, whereas the control group demonstrated a 50.7 % increase in alanine aminotransferase, unchanged aspartate aminotransferase, and only an 8.2 % decrease in alkaline phosphatase (p<0.01 between groups). At the end of the experiment (fifteenth day), aspartate aminotransferase and alkaline phosphatase activity with Iruvetol was lower than in the control by 14.0 % and 54.9 %, confirming the diagnostic and prognostic value of evaluating the enzymatic profile. The practical value of the work lies in a methodological substantiation of Iruvetol ointment local application to ensure successful treatment of accidental purulent wounds in horses.
References
1. Kulynich, S.M. (2015). The treatment of random purulent wounds of horses on the basis of chutovo stud farm «Westphalian». Scientific Progress & Innovations, 1-2, 99–101. https://doi.org/10.31210/visnyk2015.1-2.20
2. Agina, O. A., & Ihedioha, J. I. (2017). Occurrence of wounds in Nigerian horses. Journal of Applied Animal Welfare Science, 20(4), 372–380. https://doi.org/10.1080/10888705.2017.1343149
3. Kamus, L. J., Theoret, C., & Costa, M. C. (2018). Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses. Plos One, 13(11), e0206989. https://doi.org/10.1371/journal.pone.0206989
4. Leise, B. S. (2018). Topical wound medications. Veterinary Clinics of North America: Equine Practice, 34(3), 485–498. https://doi.org/10.1016/j.cveq.2018.07.006
5. Pratt, S., Sole-Guitart, A., de Klerk, K., Evans, E., Hume, J., Palmieri, C., Rainger, J., & Goodwin, W. (2024). Antinociceptive and wound healing effects of a commercial formulation of lidocaine, bupivacaine, adrenaline and cetrimide applied topically to superficial skin wounds in horses. Veterinary Record, 195(3), e4395. https://doi.org/10.1002/vetr.4395
6. Duddy, H. R., Schoonover, M. J., Williams, M. R., & Rudra, P. (2022). Healing time of experimentally induced distal limb wounds in horses is not reduced by local injection of equine-origin liquid amnion allograft. American Journal of Veterinary Research, 83(8), ajvr.21.10.0169. https://doi.org/10.2460/ajvr.21.10.0169
7. Dos Santos, T. S., Dillmann, J. B., Giovelli, M., Elias, G. O., Lima, R. K., Cescon, S., Stainki, D. R., Dos Santos, R. C., Corrêa, C., & Monteiro, S. G. (2026). Maggot therapy in horses with traumatic wounds caused by wire fences: Case reports. Journal of Equine Veterinary Science, 158, 105802. https://doi.org/10.1016/j.jevs.2026.105802
8. Textor, J. A., Clark, K. C., Walker, N. J., Aristizobal, F. A., Kol, A., LeJeune, S. S., Bledsoe, A., Davidyan, A., Gray, S. N., Bohannon-Worsley, L. K., Woolard, K. D., & Borjesson, D. L. (2018). Allogeneic stem cells alter gene expression and improve healing of distal limb wounds in horses. Stem Cells Translational Medicine, 7(1), 98–108. https://doi.org/10.1002/sctm.17-0071
9. Mund, S. J. K., MacPhee, D. J., Campbell, J., Honaramooz, A., Wobeser, B., & Barber, S. M. (2021). Macroscopic, histologic, and immunomodulatory response of limb wounds following intravenous allogeneic cord blood-derived multipent mesenchymal stromal cell therapy in horses. Cells, 10(11), 2972. https://doi.org/10.3390/cells10112972
10. Launois, T., Moor, P. L., Berthier, A., Merlin, N., Rieu, F., Schlotterer, C., Siegel, A., Fruit, G., Dugdale, A., & Vandeweerd, J. M. (2021). Use of negative pressure wound therapy in the treatment of limb wounds: A case series of 42 horses. Journal of Equine Veterinary Science, 106, 103725. https://doi.org/10.1016/j.jevs.2021.103725
11. Fowler, A. W., Gilbertie, J. M., Watson, V. E., Prange, T., Osborne, J. A., & Schnabel, L. V. (2019). Effects of acellular equine amniotic allografts on the healing of experimentally induced full-thickness distal limb wounds in horses. Veterinary Surgery, 48(8), 1416–1428. https://doi.org/10.1111/vsu.13304
12. Ludwig, E. K., & van Harreveld, P. D. (2018). Equine wounds over synovial structures. Veterinary Clinics of North America: Equine Practice, 34(3), 575–590. https://doi.org/10.1016/j.cveq.2018.07.002
13. Morales Yñiguez, F. J., & Leise, B. S. (2025). Complications in wound management. Veterinary Clinics of North America: Equine Practice, 41(3), 563–578. https://doi.org/10.1016/j.cveq.2025.08.006
14. Rajesh, A., Ju, E. D. E., Oxford, K. A., Harman, R. M., & Van de Walle, G. R. (2024). The mesenchymal stromal cell secretome promotes tissue regeneration and increases macrophage infiltration in acute and methicillin-resistant Staphylococcus aureus-infected skin wounds in vivo. Cytotherapy, 26(11), 1400–1410. https://doi.org/10.1016/j.jcyt.2024.06.007
15. Eggleston, R. B. (2018). Wound management: Wounds with special challenges. Veterinary Clinics of North America: Equine Practice, 34(3), 511–538. https://doi.org/10.1016/j.cveq.2018.07.003
16. Wise, L. M., Bodaan, C. J., Stuart, G. S., Real, N. C., Lateef, Z., Mercer, A. A., Riley, C. B., & Theoret, C. L. (2018). Treatment of limb wounds of horses with orf virus IL-10 and VEGF-E accelerates resolution of exuberant granulation tissue, but does not prevent its development. PLoS ONE, 13(5), e0197223. https://doi.org/10.1371/journal.pone.0197223
17. Kulinych, S. M., Petrenko, M. O., & Bielakova, M. A. (2018). Analiz khirurhichnoi patolohii v umovakh kinnoho zavodu "Euro Equine Saint Nicholas des Bois France" [Analysis of surgical pathology in the conditions of the horse farm "Euro Equine Saint Nicholas des Bois France"]. In Materialy II Vseukrainskoi naukovo-praktychnoi internet-konferentsii, prysviachenoi 60-richchiu z dnia narodzhennia profesora P. I. Lokesia. (pp. 18–20). Poltava. (in Ukrainian)
18. Bohdanov, V. O., & Yemelianenko, O. V. (2025). Poshyrennia, diahnostyka ta likuvannia ran u konei [Spread, diagnostics and treatment of wounds in horses]. In Vseukrainska naukova-praktychna konferentsiia zdobuvachiv vyshchoi osvity "Molod –dlia ahrarnoi nauky i vyrobnytstva". Aktualni problemy veterynarnoi medytsyny (pp. 175–177). Bila Tserkva. (in Ukrainian)
19. Zhegunov, G. F., Bayeva, T. I., & Vasilina, E. G. (2016). Metabolichnyi profil syrovatky krovi sportyvnykh konei ukrainskoi verkhovoi porody v umovakh fizychnoho ta emotsiinoho navantazhennia [Metabolic profile of blood serum of Ukrainian sport horses under conditions of physical and emotional stress]. The Journal of V. N. Karazin Kharkiv National University. Series: Biology, 27, 125–130. (in Ukrainian).
20. Prus, M. P., Shtrykul, N. S., Kurman, A. F., & Mokriy, Yu. O. (2010). Analiz biokhimichnykh pokaznykiv syrovatky krovi konei za babeziozu [Analysis of biochemical indicators of horse blood serum for babesiosis]. Visnyk Poltavskoi Derzhavnoi Ahrarnoi Akademii, 2, 101–103. (in Ukrainian).
